The Research Technician position will assist scientists in performing scientific research, development and/or clinical tasks of varying complexity. In general, this position will assist in the set up, operation and maintenance of laboratory instruments and equipment, monitoring experiments, making observations and calculating and recording results.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

The Barbie Laboratory in the Division of Molecular and Cellular Oncology and the Department of Medical Oncology at the Dana-Farber Cancer Institute/Harvard Medical School focuses on developing novel immunotherapies for lung and other cancers.

Dr. Barbie is seeking a highly motivated laboratory research technician to join a research team that works to develop novel immunotherapies using ex vivo and other approaches. This individual will work with the principal investigator to perform cutting-edge research with potential impact on cancer treatment.  The ability to perform a variety of cellular and molecular biology techniques is essential for this role, as are good organizational and communication skills, ability to work within a team, flexibility and responsibility. 

The position will require a core foundation of molecular and cellular biology with some experience in tissue culture, and willingness to work with mice.

The successful candidate will be expected to help with several projects and will be performing the following techniques/tasks.

Specific duties would include:

-Tissue culture and work with patient-derived tumor models

-Protein chemical assays including immunoblotting.

-Transfection and transduction of mammalian cell lines

-Cloning using restriction enzymes and recombination cloning

-Generation of lentiviruses and adenoviruses

-Cell proliferation assays following pharmacological treatments and genetic inactivation by CRISPR.

-Data analysis and recording of results electronically and in a lab notebook.

-Preparation of reagents and media used in lab experiments.

-Working closely with a scientist who will serve as direct supervisor and mentor.

-Ordering lab supplies

-Preparation of laboratory reagents

We are looking for an enthusiastic, capable, and competent scientist, with a BA/BS required, preferably in biology or a related field.  Preference will be given to those with a strong background in coursework in molecular and cellular biology with at least some laboratory experience in that area. In addition to technical and scientific knowledge, this individual should have good organizational and interpersonal skills and a good problem solver. Strong preference will be given to individuals that will spend 2 years as a technician.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.