POSITION SUMMARY The Research Assistant II is responsible for research support duties in support of the Principal Investigator’s (PI) studies in collaboration with the Biophotonics Core at the Salk Institute for Biological Studies. This specific position is a joint position between the laboratories of Dr. Dmitry Lyumkis and Dr. Uri Manor, bridging structural virology and microscopy / imaging, in order to visualize and structurally define the dynamics of viral infections in cells. The primary work broadly involves mammalian tissue culture, virology, and imaging. Duties include general mammalian cell culture, maintaining and passaging cells in tissue culture environment, growing and harvesting fluorescently labeled lentivirus, optimizing viral infections, and using different imaging techniques in conjunction with the Biophotonics Core facility to examine different stages of infection, all in support of multiple research projects. The ultimate goal is to use cryo-correlative techniques coupled to focused ion beam milling and cryo-electron tomography to structurally characterize viral infections. Auxiliary duties will include supervising lab staff (junior technicians, students and volunteers) and training staff in molecular biology and cell biology techniques as necessary. Incumbent will be part of a broader research team and is also responsible for working with collaborators to achieve specific research goals. GENERAL POSITION SUMMARY This job position focuses on establishing tools and workflows for fluorescence and cryo-EM based imaging, and ultimately structural characterization of the distinct events in the early stages of the lentiviral lifecycle. The incumbent works on problems in which analysis of situations or data requires an in-depth evaluation of various factors. Exercises judgement within broadly defined practices and policies in selecting methods, techniques, and evaluation criteria for obtaining results. May determine methods and procedures on new assignments. May provide supervision, training and guidance to lower level personnel. Work is done under periodic supervision. ESSENTIAL FUNCTIONS (90%) Research Support
(10%) Lab Support
EXPERIENCE Required:
Preferred:
EDUCATION Required:
Preferred:
SKILLS AND ABILITIES Required:
Preferred:
SPECIAL CONDITIONS OF EMPLOYMENT
PHYSICAL REQUIREMENTS This position requires constant handling, seeing, sitting, touching/feeling, analyzing, calculating, reasoning, and working inside.
Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities The contractor will not discharge or in any other manner discriminate against employees or applicants because they have inquired about, discussed, or disclosed their own pay or the pay of another employee or applicant. However, employees who have access to the compensation information of other employees or applicants as a part of their essential job functions cannot disclose the pay of other employees or applicants to individuals who do not otherwise have access to compensation information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in furtherance of an investigation, proceeding, hearing, or action, including an investigation conducted by the employer, or (c) consistent with the contractor’s legal duty to furnish information. 41 CFR 60-1.35(c)
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