The Research Technician position will assist scientists in performing scientific research, development and/or clinical tasks of varying complexity. In general, this position will assist in the set up, operation and maintenance of laboratory instruments and equipment, monitoring experiments, making observations and calculating and recording results.
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.
A highly motivated laboratory technician is being sought to join a research team that studies alterations in signaling proteins that contribute to oncogenesis. This individual will work with a post-doctoral fellow using cutting-edge molecular biology, genomics techniques and murine models. The environment is highly collaborative, stimulating, and multi-disciplinary, and will provide a rich environment in which to advance skills in basic cancer biology. The ability to work with live mice and to master a variety of cellular and molecular biology techniques is essential for this role, although the previous experience is not required. Laboratory technician should have good organizational and communication skills, the ability to work within a team, flexibility, and responsibility. Under the guidance of the research team, the ideal candidate will also be expected to collect data, interpret experimental results, and synthesize findings within the larger context of the project(s).
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Participate in molecular and cellular biology experiments, including but not limited to: PCR, DNA and protein gel electrophoresis, Western blots, RNA preps, quantitative real-time PCR, mammalian cell (tissue) culture, transfect and infect cells with expression vectors, CRISPR - cas9 delivery in mammalian cell (tissue) culture.
Work with murine models of lung disease relevant to molecular targets of interest, including murine colony maintenance, genotype confirmation of gene-targeted mice, and cell and molecular studies based on samples from murine models.
Routine care for laboratory colonies of mice
Maintaining accurate and up-to-date records of all animal experiments.
Maintain equipment and supplies in a neat and orderly manner.
Submit and place orders for supplies, chemicals, and other reagents.
Additional laboratory duties as needed.
Minimum of Bachelor's in Science
Experience with molecular cloning, PCR, CRISPR/Cas9 technique, and primary cell culture is desirable, but not required.
Experience with murine models is desirable, but not required
Ability to identify potential problems, communicate and troubleshoot them effectively.
Excellent written and verbal communication skills. Must be able to thoroughly document experiments, protocols, and data. Must be able to logically structure tasks and set priorities.
SKILLS/ ABILITIES/ COMPETENCIES REQUIRED:
It is essential that the technician is able to work with live mice. Previous experience is not required
Quality of work and data produced – accuracy, attention to detail, raises questions promptly with Investigators about matters affecting data quality. Ability to independently follow protocols and troubleshoot procedures as required.
Time management – meets deadlines, balances multiple projects at all times.
Communication – professional, courteous, and accurate communication of progress or results to a supervisor and principal investigator.
Excellent organizational skills and ability to prioritize tasks
Ability to use computers and enter data accurately
Ability to perform calculations accurately
Documentation – ability to provide thorough written and/or electronic documentation of protocols, weekly project status reports and regularly review with principal investigators.
Ability to work independently and to function as part of a team.
Job Qualifications –We are seeking an enthusiastic and exceptionally motivated scientist, with a BA/BS in biology or a related field, and with laboratory experience in molecular and cellular biology. In addition to technical and scientific knowledge, this individual should have excellent organizational and interpersonal skills, a working knowledge of computerized data handling, and the ability to perform meticulous record keeping. Candidates with plans to pursue cancer biology-related M.D. and/or Ph.D. degrees are strongly encouraged to apply.
Bachelor's Degree required with a major in biology or chemistry preferred.
Knowledge of information and techniques related to either biology, chemistry, or medicine is preferred.
Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff.
Ability to analyze information and use logic to address work-related issues and problems.
Must have attention to detail and be thorough in completing work tasks.
Ability to work within a team environment.
Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.
Internal Number: 2020-20659
About Dana-Farber Cancer Institute
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to under-served members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.
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