We seek a motivated Technician B to join a research team whose goal is to improve the lives of cancer patients through innovation. The technician will support studies in the interactions of immunity and cancer evolution, metastasis and drug resistance.
The laboratory uses cutting-edge technologies, including single-cell genomics, integrated CRISPR screens with single-cell readouts, and highly-multiplexed imaging, among other technologies. We work with tissue from cancer patients, cell line models and mouse models of cancer biology and cancer immunotherapy, and continue the development of novel technologies in the lab.
Responsible for preparation and maintenance of basic dry and wet chemicals and other common laboratory reagents.
Process tissue samples from preclinical animal models and patients, including cryopreservation, cell fractionation using FACS or MACS, and extraction of nucleic acids and/or proteins.
Perform conventional PCR, quantitative RT-PCR, and immunoblotting.
Perform extraction of nucleic acids and proteins from biologic specimens.
Responsible for maintenance of cell lines and primary cells in tissue culture.
Assist with in vitro therapeutic studies in cell lines and primary samples, including in vitro proliferation and metabolic luminescence assays.
Perform fluorescence- and magnetic-activated cell sorting (FACS and MACS, respectively).
Help with generation of high-throughput single-cell sequencing libraries from DNA and RNA specimens
Assist with mouse colony management.
Assist with high-content imaging and multiplexed immunofluorescence imaging on specialized equipment.
Maintain detailed records of laboratory work and communicate results over shared electronic communications platforms.
Assist with operation and maintenance of common laboratory equipment.
Perform additional related duties as assigned.
Bachelor's degree in chemistry, biology or physics or related science or equivalent in education, training and experience
Eager to learn, flexible, highly organized
Excellent communication and record-keeping abilities
The candidate selected for this position is required to undergo a drug screening test prior to a final offer of employment being made
Participation in the medical surveillance program
Work with laboratory animals
Successful completion of applicable systems training requirements
Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents.
Internal Number: 504389
About Columbia University
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.
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