Positions at this level require that skilled techniques be performed independently. Skilled procedures are defined as: those which require delicate manipulation and/or fine adjustments to equipment in response to activity of the sample or specimen being used, based upon the technician's previous experience with the equipment and/or test being performed. An example of work at the skilled level is column chromatography where the technician is expected to adjust the apparatus to achieve good separation of components of the mixture. In addition, the LST II is expected to perform general mathematical and statistical analysis of the data including: means, standard errors, t-tests and linear regression curve analysis, with the help of a desk-top calculator. The Life Science Technician II may be required to learn and perform highly skilled techniques as a small part of the job. CHARACTERISTIC DUTIES:
Animal surgical techniques such as suturing, catheterization and infusion of arteries, small organ dissection, organ perfusion and tumor implantation and passage in small animals
Immunohistological procedures including frozen sectioning and multi-step immunologic staining for fluorescence or enzymatic detection. Some level of histologic assessment may be required
Maintenance, transfer and establishment of cell lines from normal or tumor cells, preparation and bioassay of serum, screening for contamination, isolation of cell mutants, cell staining, mass production of cells and cell cloning
Fixation, dehydration, staining and embedding of specimens for electron microscopy; carbon coating, negative staining, shadowing, operation of electron microscope (EM) either transmission or scanning; use of an ultramicrotome with a diamond knife for routine thin sectioning; alignment of an EM where the system is automated
Purification and /or structural characterization of compounds for which a protocol is well established
Separation of cells, organelles, or molecules using density gradients
Radioimmunoassay of viruses, proteins, or hormone levels in cell extract according to established protocol
Techniques such as electrophoresis, chromatography, autoradiography, radiolabeling of animals, cell cultures, or macromolecules
Isolation, purification and maintenance of mammalian viruses and bacteriophages; determination of viral concentration
Genetic manipulation of bacteria including mutagenesis, phage transduction and transformation
Accurate documentation of experimental protocols and basic statistical analysis of data.
Requires 8 years of experience and practical knowledge of mouse eye histological phenotyping.
Excellent written and verbal skills with background in English instruction, scientific writing and grant editing.
Knowledge of mouse ocular anatomy.
Technical skills for microscopy, image capture, image editing.
Experience with online database.
Location: School of Medicine, California, United States Schedule: Classification Level:
Stanford is an equal opportunity employer and all qualified applicants will receive consideration without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, veteran status, or any other characteristic protected by law.
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Located between San Francisco and San Jose in the heart of Silicon Valley, Stanford University is recognized as one of the world's leading research and teaching institutions. Leland and Jane Stanford founded the University to "promote the public welfare by exercising an influence on behalf of humanity and civilization." Stanford opened its doors in 1891, and more than a century later, it remains dedicated to finding solutions to the great challenges of the day and to preparing students for leadership in a complex world. The University's thriving diverse community is comprised of nearly 7000 undergraduate students, 9000 graduate students, 2000 faculty members, 1900 postdoctoral scholars, and over 11,000 academic and administrative staff in seven schools including several interdisciplinary research centers and institutes. The campus spreads over 8000 contiguous acres and nearly all undergraduates live on campus. Stanford offers bachelor's and master's degrees in addition to doctoral degrees (PhD, MD, DMA and JD) plus a number of professional and continuing education programs and certifications. More at http://facts.stanford.edu and http://www.stanford.edu.Stanford University is an eq...ual opportunity employer and is committed to increasing the diversity of its faculty. It welcomes nominations of and applications from women, members of minority groups, protected veterans and individuals with disabilities, as well as from others who would bring additional dimensions to the university’s research, teaching and clinical missions.
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