Full Time 40 hours Grade 077 Ped Allergy/Immunology-SMD Schedule 8:30 AM-5 PM; OCC EVENINGS; RARE WEEKENDS OR HOLIDAYS; SCHEDULE MAY VARY BASED ON OPERATIONAL NEEDS Responsibilities Overview The position will provide technical support for experiments performed in an Allergy and Immunology Laboratory. . Projects in the lab focus on understanding factors predisposing to atopic diseases with an overall goal of food allergy prevention. The Technician must be able to work in a collaborative lab environment internal and external to the Division and Department. The Technician will plan and perform routine molecular and immunobiology techniques as well as technical functions including the development and maintenance of laboratory safety protocols, experimental protocols, purchase and maintenance of laboratory supplies,. The Technician will help to train and assist new laboratory personnel including undergraduate and graduate students in routine research techniques. Working with mouse models may become necessary in the future. Results from experiments performed by the Technician will be used both in publications and grant proposals. Demonstrates ICARE* values in each of the major responsibilities. Responsibilities Immunobiology Techniques Isolate blood cell fractions using density centrifugation, analyze cells by flow cytometry and immuno-cytochemistry, analyze human serum, milk, stool, and saliva using ELISA and Luminex multiplex immunoassays. Molecular Biology Techniques Develop, implement, and troubleshoot experiments utilizing molecular biology techniques, including isolation of RNA and DNA, PCR, RT_PCR, and qPCR. Cell Biology Techniques Culture immortalized cell lines and isolated human blood cells using basic cell culture techniques. General Analyze data, help to provide written summaries of data for grants and publications; develop figures for grants and publications. Use software applications to generate test reports; present results including results of literature reviews to supervisor and PI and at lab meetings with the research team. Prepare figures and tables for grant applications or to present data. Critically appraises research methodology and identifies issues or potential for issues and makes recommendations to PI and Supervisor for resolutions. Prepares basic statistical testing including graph generation. Prepare general buffers and media. Assist with maintenance, management, and/or repair of equipment. Assist with management of the laboratory's chemical inventory. Monitor and coordinates ordering of lab inventories ensuring orders for supplies as properly processed. Train individuals in the appropriate use of equipment. Responsible for orderly appearance of the laboratory. Assist to train new lab personnel and students; assist laboratory personnel, undergraduate and graduate students and technicians in the laboratory in terms of execution and troubleshooting of their experimental work including research compliance around animal care and routine research techniques. Monitor quality of day-to-day work of these individuals. With supervisor, involved in interviewing and hiring decisions; evaluating performance and resolving performance issues. Provide instruction to other laboratory personnel in BSL2 procedures and general laboratory research techniques. Assist to orient new and collaborating laboratory personnel to laboratory-related procedures. Attends weekly laboratory meetings and relevant seminars pertinent to the research goals of the laboratory. Meets with PI on a regular basis to discuss research findings and make recommendations regarding future directions. Requirements: AAS degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience. BS/BA in related field of study and 1 year experience in laboratory science is preferred. Experience working with tissue culture and flow cytometry preferred. Candidate must have excellent command of oral and written English language and able to work in a collaborative work environment. Attention to detail and meticulous ability to maintain records is essential. Advanced computer skills including MS Word and Excel, required. Experience using flow cytometry software, statistical software packages such as Prism are preferred. Occasional evening or weekend hours may be necessary. Schedule varies based on operational needs. Competencies: Knowledge of general laboratory safety processes, universal precautions, aseptic cell culture technique, handling of mice and practice of rodent euthanasia required. Thorough documentation of results in written and electronic database formats will be required. Skills in basic database management and generation of publication-quality figures preferred. Needs to be proficient in anesthetic techniques.
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