Full Time 40 hours Grade 077 Ctr Musculoskeletal Research Schedule 8 AM-4:30 PM Responsibilities Position Summary: This is a Technical position within the Eliseev and Jonason Laboratories in the Center for Musculoskeletal Research. The job consists of laboratory work using complex genetic mouse models and primary cell culture systems to understand the role of mitochondria in osteoarthritis. This position requires mouse handing (mating, weaning, etc.), mouse recovery surgery, tissue harvest and sample processing (preparation of mouse tissues for histology and microCT analyses), molecular biology skills (PCR, gel electrophoresis, Western blotting, etc.), cell culture skills (mammalian cell culture, transfection), data analysis/interpretation, excellent organizational skills, and careful attention to detail. Responsibilities: Breed, maintain, and genotype mice and keep detailed electronic inventory records of the mouse colonies. Perform surgical procedures such as the meniscal/ligamentous injury and the tibia/femur fracture for the support of ongoing research projects. Dissection of postnatal mouse skeletal tissues, sectioning of paraffin or frozen tissues, histological staining, histomorphometry, and immunohistochemistry of samples. Isolation, culture, adenoviral/lentiviral infection, and transient transfection of primary skeletal cells and cell lines. Isolation and analysis of RNA and protein from cell cultures via real time quantitative PCR and Western blotting, respectively. Maintenance of a detailed lab notebook with experimental detail, methods, data compilation and data analysis and interpretation included. Requirements Associate's degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience. 2 years of experience with mouse work and cell culture is preferred. Additional specific training will be provided.
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